Bioprocess development to enhance biomass and lutein production from Chlorella sorokiniana Kh12

Present study focused on optimizing bioprocess condition for microalgal lutein production. From previous baseline yields of biomass (3.46 g/L) and lutein (13.7 mg/g), this study examined few key parameters. The 3X:3X ratio macro-and micronutrients was the most affecting parameter with highest biomass and lutein yields of 4.61 g/L and 14.3 mg/g. Temperature 30 degrees C enhanced the lutein up to 17.3 mg/g but reduced the biomass to 3 g/L. The light effects study showed 10 k lux was most effective for lutein up to 14 mg/g, and effect of increasing salinity (25-75 %) was detrimental. All the above parameters' optimization resulted in a lipid content of 22.5-26.5 %. A maximum lutein productivity and yield of 0.451 mg/L/d and 65.74 mg/L with a 3X:3X macro -and micronutrient ratio was achieved. The Chlorella sorokiniana Kh12 strain exhibited one of the highest yields among recent reports; hence it could be a source for commercial lutein production.

Automated summary

This study aimed to optimize the bioprocess condition for microalgal lutein production. The 3X:3X ratio macro-and micronutrients showed the highest impact on biomass and lutein yields. Temperature at 30 degrees C increased lutein production but reduced biomass. An optimal light intensity of 10 k lux and a lower salinity level were beneficial for lutein production. The Chlorella sorokiniana Kh12 strain exhibited high lutein yields and has potential for commercial production.