Clostridium kluyveri enhances caproate production by synergistically cooperating with acetogens in mixed microbial community of electro-fermentation system

As a chain elongation (CE) model strain, Clostridium kluyveri has been used in the studies of bioaugmentation of caproate production. However, its application in the novel electro-fermentation CE system for bioaugmentation is still unclear. In this study, the CE performances, with or without bioaugmentation and in conventional or electro-fermentation systems were compared. And the mechanism of electrochemical-bioaugmentation by constructing a co-culture of Acetobacterium woodii and Clostridium kluyveri were further verified. Results demonstrated that the bioaugmentation treatments have better CE performance, especially in electro-fermentation system, with a highest caproate concentration of 4.68 g.L-1. Mechanism analysis revealed that C. kluyveri responded to the electric field and emerged synergy with the acetogens, which was proved by the increases of C. kluyveri colonization and the acetogens abundance in biofilm and supported by the co-culture experiment. This study provides a novel insight of microbial synergy mechanism of C. kluyveri during CE bioaugmentation in electro-fermentation system.

Automated summary

This study compared the performances of Clostridium kluyveri, a chain elongation (CE) model strain, with or without bioaugmentation and in conventional or electro-fermentation systems. The results showed that bioaugmentation treatments, especially in electro-fermentation system, had better CE performance, with a highest caproate concentration of 4.68 g.L-1. Mechanism analysis revealed that C. kluyveri responded to the electric field and emerged synergy with the acetogens, which was supported by the increases of C. kluyveri colonization and the acetogens abundance in biofilm.