期刊
JOURNAL OF NEUROSCIENCE
卷 36, 期 31, 页码 8070-8077出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.3393-15.2016
关键词
epilepsy; extrasynaptic; GABA receptor; neurosteroid; tonic inhibition; zinc
资金
- National Institutes of Health [R01NS051398]
- Texas Brain and Spine Institute
Zinc (Zn2+) is an essential cofactor in mammalian cells and neurons. Zn2+ is released from synaptic vesicles of certain nerve terminals in the hippocampus during neuronal activity. Zn2+ has been shown to inhibit synaptic GABA(A) receptors and alter the hippocampal network excitability. However, the ability of Zn2+ to block extrasynaptic receptors remains unclear. Endogenous neurosteroids, such as allopregnanolone (AP), regulate neuronal excitability by allosteric activation of synaptic and extrasynaptic GABAA receptors. Neurosteroids activate extrasynaptic delta GABA(A) receptor-mediated tonic inhibition in dentate gyrus granule cells (DGGCs), thereby contributing to the regulation of downstream circuit excitability. Here we report a novel inhibitory role of Zn2+ at neurosteroid-sensitive, extrasynaptic delta GABA(A) receptors by electrophysiological recordings in DGGCs from adult mice. Zn2+ displayed a concentration-dependent, reversible noncompetitive blockade of AP-sensitive tonic current in DGGCs (IC50, 16 mu M). Tonic current was fully blocked by Zn2+, akin to the GABA(A) receptor antagonist gabazine. Zn2+ inhibition of tonic current was lacking in DGGCs from delta-subunit knock-out mice. Moreover, AP-activated synaptic receptor-mediated phasic currents were not affected by Zn2+. Finally, intrahippocampal infusion of Zn2+ elicited rapid epileptiform activity and significantly blocked the antiseizure activity of APin the kindling model of epilepsy. Thus, Zn2+ inhibition of neurosteroid-sensitive, extrasynaptic GABAA receptors in the hippocampus has direct implications in many brain hyperexcitability conditions, such as seizures, epileptogenesis, and epilepsy. Zn2+ interactions may aid to further understand the physiology of extrasynaptic GABA(A) receptors.
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