Solid lipid nanoparticles (SLNs), the potential novel vehicle for enhanced in vivo efficacy of hesperidin as an anti-inflammatory agent

Hesperidin (C28H34O15), a flavanone glycoside abundantly present in citrus fruits, has proven therapeutic effects including anti-inflammatory activities. Herein, we report a novel formulation of HESP loaded solid lipid nano-particles (SLNs) using hot homogenization and ultrasound to improve the poor solubility and bioavailability. In the present study, the formulation was developed and optimized by response surface method and then charac-terized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy (FT-IR), and dynamic light scattering (DLS). Encapsulation efficiency was determined and the anti-inflammatory effect was assessed through in vivo ear edema inflammation model. According to the electron microscopy results, the product has a spherical shape. The optimized parameters produced small size (179.8 +/- 3.6 nm) HESP-SLNs with high encapsulation efficiency (93.0 +/- 3.8 %). The outcomes exhibited that encapsulation in SLNs carriers im-proves the anti-inflammatory potential of HESP.

Automated summary

In this study, a novel formulation of HESP loaded solid lipid nanoparticles (SLNs) was developed to improve the solubility and bioavailability. The optimized formulation showed high encapsulation efficiency and small size, and exhibited improved anti-inflammatory potential of HESP.