期刊
BIOMEDICAL MATERIALS
卷 18, 期 2, 页码 -出版社
IOP Publishing Ltd
DOI: 10.1088/1748-605X/acb7bf
关键词
decellularized; organ; scaffolds; decellularized organ; human pluripotent stem cell
Diabetes is a global epidemic affecting more than 285 million people worldwide. Engineering endocrine pancreas tissue using regenerative cell source shows promise for diabetes therapy. Researchers demonstrate the feasibility of re-engineering decellularized organ scaffolds using human pluripotent stem cells (hPSC) to repopulate these scaffolds. The study finds that hPSCs cultured and differentiated as aggregates are more suitable for repopulation, especially in decellularized liver scaffolds, which also support the endocrine cell fate of the engrafted cells. This novel strategy has potential applications in preclinical testing, drug discovery, and diabetes therapy.
Diabetes is an emerging global epidemic that affects more that 285 million people worldwide. Engineering of endocrine pancreas tissue holds great promise for the future of diabetes therapy. Here we demonstrate the feasibility of re-engineering decellularized organ scaffolds using regenerative cell source. We differentiated human pluripotent stem cells (hPSC) toward pancreatic progenitor (PP) lineage and repopulated decellularized organ scaffolds with these hPSC-PP cells. We observed that hPSCs cultured and differentiated as aggregates are more suitable for organ repopulation than isolated single cell suspension. However, recellularization with hPSC-PP aggregates require a more extensive vascular support, which was found to be superior in decellularized liver over the decellularized pancreas scaffolds. Upon continued culture for nine days with chemical induction in the bioreactor, the seeded hPSC-PP aggregates demonstrated extensive and uniform cellular repopulation and viability throughout the thickness of the liver scaffolds. Furthermore, the decellularized liver scaffolds was supportive of the endocrine cell fate of the engrafted cells. Our novel strategy to engineer endocrine pancreas construct is expected to find potential applications in preclinical testing, drug discovery and diabetes therapy.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据