期刊
BIOLOGICAL TRACE ELEMENT RESEARCH
卷 201, 期 10, 页码 4697-4709出版社
SPRINGERNATURE
DOI: 10.1007/s12011-023-03574-7
关键词
Green Synthesis; ZnO NPs; THP-1; Cytotoxicity; Apoptosis
This study investigated the effects of novel zinc oxide nanoparticles synthesized using Papaver somniferum leaf on oxidative stress, necrosis, and apoptosis in THP-1 cells. The nanoparticles showed an irregular spherical morphology with a size ranging from 20 to 30 nm. They inhibited cell proliferation in a concentration-dependent manner and induced necrosis and apoptosis in the cancer cell line. The nanoparticles also increased the formation of intracellular ROS, which is important for deactivating leukemic cell development.
This study investigated the effect of novel zinc oxide nanoparticles (ZnO NPs) biosynthesized employing Papaver somniferum leaf on oxidative stress, necrosis, and apoptosis in the leukemia cancer THP-1 cell. The obtained ZnO was examined using SEM, AFM, and TEM microscopy, which revealed an irregular spherical morphology with a size ranging from 20 to 30 nm, and the UV-vis absorbance revealed a strong absorption peak in the range of 360-370, nm confirming the production of ZnO NPs. THP-1 cells were subjected to an MTT, an EdU proliferation, a lactate dehydrogenase release tests, a reactive oxygen species (ROS) induction experiment, a DAPI staining detection assay, and a flow cytometric analysis for Annexin V to measure the effects of ZnO NPs on cancer cell growth inhibition, apoptosis, and necrosis. Our results show that ZnO NPs inhibit THP-1 line in a concentration-dependent pattern. It was observed that ZnO NPs triggered necrosis (cell death) and apoptosis in the cell line. ZnO NPs massively improved the formation of intracellular ROS, which is crucial in deactivating the development of leukemic cells. In conclusion, ZnO nanoparticles synthesized using Papaver somniferum extract have the ability to inhibit proliferation leukemic cancer cells, making them potential anticancer agents.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据