Diversity in Elemental Content in Selected Artemisia L. (Asteraceae) Species from Gilgit-Baltistan Region of Pakistan Based on Inductively Coupled Plasma Atomic Emission Spectrophotometry (ICP-AES)

Diversity in eleven Artemisia species from northern Pakistan was assessed based on as per suitability of their elemental contents with thermal conductivity detection and ICP-AES procedures. Results indicated the presence of 13 major elements in the Artemisia species with varied concentrations including Carbon (45.7%, 45,7000 ppm-49.8%, 49,8000 ppm), Nitrogen (2.03%, 20,300 ppm-3.50%, 35,000 ppm), Phosphorus (0.168%, 1680 ppm-0.642%, 6420 ppm), Potassium (2.38%, 23,800 ppm-4.72%, 47,200 ppm), Sulphur (1920 ppm, 0.192%-4780 ppm, 0.478%), Boron (23.8 ppm, 0.00238%-71.7 ppm, 0.00717%), Calcium (0.733%, 7330 ppm-2.249%, 22,490 ppm), Magnesium (0.116%, 1160 ppm-0.267%, 2670 ppm), Zinc (27.7 ppm, 0.00277%-47.9 ppm, 0.00479%), Manganese (25.7 ppm, 0.00257%-93.8 ppm, 0.00938%), Iron (353 ppm, 0.0353%-1532 ppm, 0.1532%), Copper (14.1 ppm, 0.00141%-26.2 ppm, 0.00262%) and Sodium (105 ppm, 0.0105%-587 ppm, 0.0587%). Cluster analysis distributed the Artemisia species into two major groups (G1 and G2) on the basis of their elemental content where G1 contained species like, Artemisia herba alba Asso., A. tournefortiana Rachb., A. rutifolia Steph. ex Spreng., and A. vulgaris L., with the presence of all elements with the maximum amount of S, Zn, P, Ca, and Mg, while G2 contained species like Artemisia biennis Willd., A. chamaemelifolia Vill., A. capillaris, L., A. gmelinii Weber ex Stech., A. indica Willd., A. maritima L., and A. verlotiorum Lamotte., with all elements but significant concentrations of B, N, C, K, Mn, Fe, Cu, and Na. PCA analysis displayed maximum species diversity in the axes two, while axes one showed lower diversity. Additionally, the elevated levels of elements recorded as compared to the threshold levels recommended in the literature for medicinal plants require extraordinary precautionary measures before or during using Artemisia as medication to avoid metal toxicity.

Automated summary

Diversity in eleven Artemisia species from northern Pakistan was assessed based on elemental contents. Cluster analysis divided the species into two groups, while PCA analysis revealed maximum species diversity in the second axis. The study highlights the importance of precautionary measures to avoid metal toxicity when using Artemisia as medication.