Design of optoelectrodes for the remote imaging of cells and in situ electrochemical detection of neurosecretory events

Optical fibers have opened avenues for remote imaging, bioanalyses and recently optogenetics. Besides, mini-aturized electrochemical sensors have offered new opportunities in sensing directly redox neurotransmitters. The combination of both optical and electrochemical approaches was usually performed on the platform of micro-scopes or within microsystems. In this work, we developed optoelectrodes which features merge the advantages of both optical fibers and microelectrodes. Optical fiber bundles were modified at one of their extremity by a transparent ITO deposit. The electrochemical responses of these ITO-modified bundles were characterized for the detection of dopamine, epinephrine and norepinephrine. The analytical performances of the optoelectrodes were equivalent to the ones reported for carbon microelectrodes. The remote imaging of model neurosecretory PC12 cells by optoelectrodes was performed upon cell-staining with common fluorescent dyes: acridine orange and calcein-AM. An optoelectrode placed by micromanipulation at a few micrometers-distance from the cells offered remote images with single cell resolution. Finally, in situ electrochemical sensing was demonstrated by additions of K+-secretagogue solutions near PC12 cells under observation, leading to exocytotic events detected as amperometric spikes at the ITO surface. Such dual sensors should pave the way for in vivo remote imaging, optogenetic stimulation, and simultaneous detection of neurosecretory activities.

Automated summary

This study developed optoelectrodes that combine the advantages of optical fibers and microelectrodes, enabling remote imaging, optogenetic stimulation, and simultaneous detection of neurosecretory activities. The optoelectrodes demonstrated analytical performance equivalent to carbon microelectrodes and allowed for remote imaging of single cells and in situ electrochemical sensing.