期刊
BIOCHEMISTRY-MOSCOW
卷 87, 期 11, 页码 1301-1309出版社
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S0006297922110098
关键词
proteogenomics; shotgun proteomics; zebrafish; ADAR; RNA-dependent adenosine deaminase; RNA editing
资金
- Russian Science Foundation [20-15-00072]
RNA editing can lead to protein recoding, and recoding sites were identified in zebrafish proteins for the first time. This could have functional consequences for the proteins in the zebrafish.
RNA editing by adenosine deaminases of the ADAR family can lead to protein recoding, since inosine formed from adenosine in mRNA is complementary to cytosine; the resulting codon editing might introduce amino acid substitutions into translated proteins. Proteome recoding can have functional consequences which have been described in many animals including humans. Using protein recoding database derived from publicly available transcriptome data, we identified for the first time the recoding sites in the zebrafish shotgun proteomes. Out of more than a hundred predicted recoding events, ten substitutions were found in six used datasets. Seven of them were in the AMPA glutamate receptor subunits, whose recoding has been well described, and are conserved among vertebrates. Three sites were specific for zebrafish proteins and were found in the transmembrane receptors astrotactin 1 and neuregulin 3b (proteins involved in the neuronal adhesion and signaling) and in the rims2b gene product (presynaptic membrane protein participating in the neurotransmitter release), respectively. Further studies are needed to elucidate the role of recoding of the said three proteins in the zebrafish.
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