Inhibition of Klebsiella pneumoniae carbapenemases by mycocins produced by Wickerhamomyces anomalus

New alternatives have been under study as treatment due to the problem of multidrug-resistant bacteria. Among them, Wickerhamomyces anomalus mycocins have shown a great potential against several microorganisms since they have high antimicrobial activity, as well as they can be used as fast available nutrients and stand several extreme conditions. In this way, Klebsiella pneumoniae carbapenemases inhibition by mycocins produced by W. anomalus is important. Microdilution assays were carried out to evaluate strains inhibition in liquid medium and the test in solid medium were carried out. Toxicity was evaluated by both hemolysis and Artemia salina Leach tests. W. anomalus supernatant showed 2.36 U/mg beta-glucanases activity, and antimicrobial activity was evidenced in 100% Klebsiella pneumoniae carbapenemase strains up to 0.12 U/mg concentration. Besides, there was low toxicity in hemolysis and Artemia salina Leach tests. It is suggested that W. anomalus mycocins may be an alternative to develop new antimicrobial substances.

Automated summary

Wickerhamomyces anomalus mycocins show high antimicrobial activity and the ability to withstand extreme conditions, making them important for inhibiting Klebsiella pneumoniae carbapenemases. The experimental results demonstrate that W. anomalus mycocins have antimicrobial activity against Klebsiella pneumoniae carbapenemase strains and low toxicity.