Paracoccus marinaquae sp. nov., isolated from coastal water of the Yellow Sea

A Gram-stain-negative, non-motile and coccoid bacterial strain, designated XHP0099T, was isolated from the coastal water of the Yellow Sea, China. Growth occurred at 20-37 degrees C (optimum, 28 degrees C), pH 5.0-9.0 (optimum, pH 7.0-8.0), and with 0-7.0% NaCl (optimum, 2.0-3.0%). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain XHP0099T was related to members of the genus Paracoccus and shared the highest sequence similarity with P. siganidrum M26 (98.2%), followed by P. alkanivorans 4-2 (T) (97.6%) and P. alkenifer DSM 11593 (T) (97.4%). The average nucleotide identity, amino acid identity, and digital DNA-DNA hybridization values of strain XHP0099(T) against related members in the genus Paracoccus were below the cut-off points proposed for the delineation of a novel species. The major cellular fatty acids (> 10%) were summed feature 8 (C-18:1 omega 7c/C-18:1 omega 6c), and C-18:0. The major isoprenoid quinone was Q-10 and the polar lipids contained diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylcholine (PC), aminolipid (AL) and unidentified polar lipids (L). The G + C content of the genomic DNA of strain XHP0099T was 66.0%. Genomic analysis suggested that strain XHP0099(T) harbored gene clusters for formaldehyde and the XoxF-type methanol oxidation and type 1 Calvin cycle, which could confer the methylotrophy pathway. Based on the phenotypic, phylogenetic, biochemical and chemotaxonomic analysis, strain XHP0099T represents a novel species of the genus Paracoccus, for which the name Paracoccus marinaquae sp. nov. is proposed. The type strain is XHP0099T (= JCM 34661 (T) = GDMCC 1.2414 (T) = MCCC 1K05846(T)).

Automated summary

A new species of genus Paracoccus, named Paracoccus marinaquae sp. nov., was isolated from the coastal water of the Yellow Sea, China. This bacterium is Gram-stain-negative, non-motile and coccoid. It grows at a temperature range of 20-37 degrees C, pH range of 5.0-9.0, and NaCl concentration range of 0-7.0%. Based on phylogenetic analysis and phenotypic characteristics, it was determined to be a novel species within the genus Paracoccus.