期刊
AQUACULTURE
卷 562, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aquaculture.2022.738804
关键词
Ichthyophthirius multifiliis; White spot disease; Intraspecific variation; Genetic diversity; Epidemic investigation
This study aimed to compare the epidemic information of different I. multifiliis isolates in China and determine their genetic diversity. The results showed significant genetic variation among the 16 isolates, which were classified into seven genotypes. Furthermore, analysis of the 40 reported isolates confirmed the existence of at least three genetic groups in China. These findings demonstrate a high level of genetic diversity in I. multifiliis in China.
Ichthyophthirius multifiliis (Fouquet, 1876) is a highly pathogenic ciliate of freshwater fish that threatens the global aquaculture. During the investigations of ichthyophthiriasis in China, a total of sixteen I. multifiliis isolates were collected from four provinces, Hubei, Hunan, Guangdong, and Shanghai. The purpose of this study was to compare the epidemic information of the different isolates and determine the genetic diversity of I. multifiliis in China. The clinical signs and histopathological changes caused by I. multifiliis infection were presented in all the collected fish samples and no obvious differences were observed among the fish or tissues. However, significant genetic variation was found in the cytochrome c oxidase subunit I (cox-1, 0.07%-2.19%,) and NADH dehydro-genase subunit I (nad1_b, 0.00%-3.23%) sequences of the sixteen isolates. Based on the concatenated cox-1 and nad1_b sequences, all the sixteen isolates were defined as novel genotypes. The phylogenetic analyses of these genotypes suggested at least three genetically distinct groups exist in China. This was confirmed by further analyses, which divided the all the forty reported I. multifiliis isolates into nine genetic groups, while the isolates from this study were distributed among seven of these groups. Additionally, isolates from the same sources shared a closer phylogenetic relationship. However, some groups, containing isolates from China, the United States, and Turkey, also reflected the frequently interaction of I. multifiliis between these countries. These data demonstrated a higher level of genetic diversity in I. multifiliis in China, which facilitates a better understanding of the migration and evolution of this parasite.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据