期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 107, 期 1, 页码 111-123出版社
SPRINGER
DOI: 10.1007/s00253-022-12298-y
关键词
Silver nanoparticles; Dengue virus; Alocasia odora; Cytotoxicity; Anti-viral activity
In this study, silver nanoparticles (AgNPs) were successfully synthesized using the aqueous extract of Alocasia odora plant. The cytotoxicity of the AgNPs was higher than the extract alone on U87-MG human glioblastoma cancer cell line, and the nanoparticles also showed significant reduction in dengue viral infection compared to the extract alone. These findings suggest that AgNPs can be potential antiviral drug candidates.
Silver nanoparticles (AgNPs) have various applications in the biomedical field and are considered excellent microbicidal agents. Moreover, biological synthesis of AgNPs using medicinal plants further improves the medicinal applicability of these plants. In this study, the aqueous extract of Alocasia odora rhizome (RE) and Alocasia odora stem (SE) were used to synthesize stem aqueous extract-AgNPs (SNP) and rhizome aqueous extract-AgNPs (RNP). Furthermore, RNP and SNP were evaluated for their virucidal potential. The synthesis of SNP and RNP was monitored using a UV spectrophotometer by observing their surface plasmon resonance peak. In addition, scanning electron microscopy (SEM) gave further insight into their morphology and particle size, whereas energy-dispersive X-ray spectroscopy (EDX) confirmed the presence of silver ions. Interestingly, Fourier-transform infrared spectroscopy (FTIR) analysis of AgNPs revealed that phytomolecules acted as capping and stabilizing agents for SNP and RNP. The in vitro cytotoxicity of SNP and RNP was further analyzed using MTT assay on the U87-MG human glioblastoma cancer cell line and SNP found to be the most cytotoxic (43.40 mu g/ml) among all. Besides that, SNP has also found to show the maximum cytopathic effects (CPE) against dengue virus type 2 (DENV-2) on Huh-7 cell line. As a result of the observations, it can be concluded that they can become a promising antiviral drug candidate and thus merit further testing. Key points AgNPs were successfully synthesized through Alocasia odora aqueous extract. AgNPs were more cytotoxic on the U87-MG cell line than the extract alone. AgNPs have shown significant reduction in the dengue viral infection than the extract alone.
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