4.7 Article

Engineering D-glucose utilization in Azospirillum brasilense Sp7 promotes rice root colonization

期刊

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 106, 期 23, 页码 7891-7903

出版社

SPRINGER
DOI: 10.1007/s00253-022-12250-0

关键词

Azospirillum; D-glucose utilization; Phosphotransferase system; Rice root colonization

资金

  1. Indian Council of Agricultural Research (ICAR), New Delhi
  2. J C Bose National Fellowship (SERB)
  3. Senior Research Associateship (SRA) from the Council of Scientific and Industrial Research (CSIR), New Delhi

向作者/读者索取更多资源

This study identified the genes responsible for D-glucose utilization ability in A. brasilense Sp7 by cloning and comparing genomes. The transcriptional fusion of these genes improved the D-glucose utilization ability and root colonization of rice seedlings.
Bacteria of the genus Azospirillum include several plant associated bacteria which often promote the growth of their host plants. Although the host range of Azospirillum brasilense Sp7 is much wider than its close relative Azospirillum lipoferum 4B, it lacks the ability to efficiently utilize D-glucose for its growth. By comparing the genomes of both the species, the genes of A. lipoferum 4B responsible for conferring D-glucose utilization ability in A. brasilese Sp7 were identified by cloning individual or a combination of genes in a broad host range expression vector, mobilizing them in A. brasilense Sp7 and examining the ability of exconjugants to use D-glucose as sole carbon source for growth. These genes also included the homologs of genes involved in N-acetyl glucosamine utilization in Pseudomonas aeruginosa PAO1. A transcriptional fusion of the 5 genes encoding glucose-6-phosphate dehydrogenase and 4 components of glucose phosphotransferase system were able to improve D-glucose utilization ability in A. brasilense Sp7. The A. brasilense Sp7 strain engineered with D-glucose utilization ability showed significantly improved root colonization of rice seedling. The improvement in the ability of A. brasilense Sp7 to colonize rice roots is expected to bring benefits to rice by promoting its growth.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据