期刊
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
卷 66, 期 12, 页码 -出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/aac.01056-22
关键词
ATP synthesis; bioenergetics; F-ATP synthase; nontuberculous mycobacteria; OXPHOS; tuberculosis
资金
- National Research Foundation (NRF) Singapore, NRF Competitive Research Program (CRP)
- [NRF-CRP18-2017-01]
The F1FO-ATP synthase is essential for the survival of tuberculosis and nontuberculous mycobacteria and has been confirmed as a potential drug target. Cryo-EM structures of Mycobacterium smegmatis F-1-ATPase and F1FO-ATP synthase with different nucleotide occupancy were determined, revealing critical elements for ATP hydrolysis and synthesis. Mutational studies identified the extended C-terminal domain of subunit alpha as the main element for the self-inhibition mechanism of ATP hydrolysis. Rotation studies showed a rapid transition from the inhibition state to the active state. The mycobacterial-specific elements alpha, gamma, and delta were found to be important for ATP formation, suggesting they could be targeted for species-specific inhibitors.
The F1FO-ATP synthase is required for the viability of tuberculosis (TB) and nontuberculous mycobacteria (NTM) and has been validated as a drug target. Here, we present the cryo-EM structures of the Mycobacterium smegmatis F-1-ATPase and the F1FO-ATP synthase with different nucleotide occupation within the catalytic sites and visualize critical elements for latent ATP hydrolysis and efficient ATP synthesis. Mutational studies reveal that the extended C-terminal domain (alpha CTD) of subunit alpha is the main element for the self-inhibition mechanism of ATP hydrolysis for TB and NTM bacteria. Rotational studies indicate that the transition between the inhibition state by the alpha CTD and the active state is a rapid process. We demonstrate that the unique mycobacterial gamma-loop and subunit delta are critical elements required for ATP formation. The data underline that these mycobacterium-specific elements of alpha, gamma, and delta are attractive targets, providing a platform for the discovery of species-specific inhibitors.
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