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Article
Chemistry, Multidisciplinary
Bhumrapee Eiamthong et al.
Summary: We report the discovery of a new PET hydrolase, named MG8, from the human saliva metagenome. MG8 exhibits robust PET degradation activities under different conditions and outperforms other hydrolases in degrading PET. Furthermore, we genetically modified MG8 to serve as a covalent binder for PET functionalization, enabling the attachment of protein cargos to PET and other polyester plastics.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Isuru Karunatillaka et al.
Summary: A study investigated a PETase enzyme from environmental samples and its distribution in other bacterial communities, identifying similar homologs in JGI metagenomic datasets through BLAST searches. By verifying the protein sequences and conducting phylogenetic analyses, it confirmed that these proteins are putative PETases, and docking analysis further confirmed their functional assignment.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2022)
Article
Multidisciplinary Sciences
Hongyuan Lu et al.
Summary: Plastic waste is an ecological challenge, and enzymatic degradation offers a potentially green and scalable route for plastic recycling. Engineered PETase shows superior activity in hydrolyzing postconsumer PET, and a closed-loop recycling process is demonstrated.
Article
Chemistry, Physical
Lara Pfaff et al.
Summary: Thermophilic polyester hydrolases (PES-H) play a crucial role in the biocatalytic recycling of synthetic polyester polyethylene terephthalate (PET). By studying the crystal structures of two enzymes derived from metagenomes, researchers gained insights into the mechanism of enzymatic PET hydrolysis. Mutations in key residues resulted in improved hydrolytic activity of certain variants, making them potential candidates for industrial plastic recycling processes.
Article
Chemistry, Physical
Elizabeth L. Bell et al.
Summary: The recent discovery of IsPETase, a hydrolytic enzyme that can degrade polyethylene terephthalate (PET), has sparked interest in using biocatalytic approaches to recycle plastics. In this study, we describe an automated, high-throughput directed evolution platform for engineering polymer degrading enzymes. By applying catalytic activity under elevated temperatures, a thermostable IsPETase variant (HotPETase) was successfully engineered to operate at the industrial processing temperature of PET. This HotPETase enzyme showed improved efficiency in degrading PET compared to previously reported enzymes and could selectively breakdown the PET component of multi-materials.
Article
Chemistry, Physical
Yinglu Cui et al.
Summary: This study successfully improved the robustness of PETase through a computational strategy, resulting in the redesign of a variant DuraPETase with significantly elevated melting temperature and enhanced degradation of PET films, as well as complete biodegradation of microplastics at mild temperatures. The design strategy presents opportunities for handling uncollectable PET waste and converting resulting monomers into high-value molecules.
Article
Chemistry, Multidisciplinary
Joanna C. Sadler et al.
Summary: This study reports a novel approach to directly upcycle post-consumer PET waste into valuable vanillin using engineered Escherichia coli. After process optimization, a conversion rate of 79% from terephthalic acid to vanillin was achieved, marking a significant improvement in yield. This work demonstrates the first biological upcycling of post-consumer plastic waste into vanillin, showcasing the potential for sustainable solutions in the plastic recycling industry.
Article
Multidisciplinary Sciences
V. Tournier et al.
Article
Chemistry, Physical
Hyeoncheol Francis Son et al.
Review
Biotechnology & Applied Microbiology
Fusako Kawai et al.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Dominik Danso et al.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2018)
Editorial Material
Multidisciplinary Sciences
Uwe T. Bornscheuer
Article
Multidisciplinary Sciences
Shosuke Yoshida et al.
Article
Biotechnology & Applied Microbiology
Sintawee Sulaiman et al.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2012)