Inner filter effect-based red-shift and fluorescence dual-sensor platforms with sulfur quantum dots for detection and bioimaging of alkaline phosphatase

Alkaline phosphatase (ALP), one of the vital biomarkers in several diseases, plays a role in indicating disease presence or severity in early diagnosis. Here, a simple H2O2 assisted top-down method was used to synthesize sulfur quantum dots (SQDs) with excitation and emission at 355 nm and 440 nm. Adding ALP into p-nitrophenyl phosphate (p-NPP) and SQDs was found to exhibit a red shift in the emission wavelength and fluorescence intensity quenching of SQDs, respectively, allowing us to propose dual-sensor platforms of red shift of emission wavelength (RSEW) and fluorescence quenching of SQDs. These dual-sensor platforms were highly sensitive and selective in ALP detection, with a linear response to ALP in the concentration range of 0.25 to 100 U L-1 and detection limits of 0.08 and 0.10 U L-1, respectively. The absorption of p-NP at 400 nm showed a good overlap with the excitation and emission of SQDs, leading to inner filter effect-based RSEW and fluorescence quenching of SQDs. This sensor platform was successfully applied in ALP sensing of serum samples as well as monitoring of ALP in cells. More importantly, this platform can serve as an example of using RSEW to detect ALP.

Automated summary

In this study, sulfur quantum dots (SQDs) were synthesized using a simple H2O2 assisted top-down method and applied in alkaline phosphatase (ALP) detection. By measuring the red shift in emission wavelength and fluorescence intensity quenching, a dual-sensor platform based on red shift of emission wavelength (RSEW) and fluorescence quenching of SQDs was successfully constructed. The platform exhibited high sensitivity and selectivity towards ALP and was able to detect ALP in serum samples and cells.