Electrochemical Determination of Homocysteine Using Self-Assembled 6-Ferrocenylhexanethiol on a Molybdenum Disulfide Nanoparticle Modified Glassy Carbon Electrode (GCE)

The determination of homocysteine (Hcy) is important because high levels are associated with a greater risk of developing heart disease or stroke. Herein, an electrochemical sensor of homocysteine was constructed based upon the self-assembly of 6-ferrocenylhexanethiol (FcC(6)SH) on a MoS2 nanoparticles-modified electrode with a Nafion coating. The sensor showed a favorable electrocatalytic activity for oxidation of homocysteine since 6-ferrocenylhexanethiol acted as a good electron transfer mediator. Moreover, the MoS2 nanoparticles served as modifiers to covalently immobilize FcC(6)SH and enhance the electrochemical active surface area. Highly sensitive determination of homocysteine was achieved with a linear range from 2.0 to 100 mu mol L-1 and a minimum detectable concentration of 0.30 mu mol L-1 by chronoamperometry. The constructed sensor was applied to the analysis of immunoglobulin G (IgG) samples with satisfactory results.

Automated summary

An electrochemical sensor was developed for the determination of homocysteine. The sensor showed improved catalytic activity and sensitivity through self-assembly and modification techniques. Satisfactory results were obtained in the analysis of immunoglobulin G samples using the sensor.