期刊
ANALYTICAL CHEMISTRY
卷 94, 期 50, 页码 17692-17699出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.2c04614
关键词
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资金
- National Key Research and Development Program of China
- National Natural Science Foundation of China
- Hubei Province Natural Science Foundation
- [2021YFD1700103]
- [21837001]
- [21977036]
- [U20A2038]
- [2020BHB027]
Bacterial plant diseases pose a serious threat to human food security, causing significant reduction in agricultural yields and economic loss. A new fluorescent sensor targeting Nitroreductase (NTR) was discovered and applied to the imaging of zebrafish and pathogenic bacteria. Importantly, this sensor enabled real-time diagnosis of bacteria-infected Brassica napus L., providing a promising tool for examining temporal and spatial infection of plant pathogens in precision agriculture.
Plant diseases caused by bacteria have become one of the serious problems that threaten human food security, which led to the remarkable reduction of agricultural yields and economic loss. Nitroreductase (NTR), as an important biomarker, is highly expressed in bacteria, and the level of NTR is closely related to the progression of pathogen infection. Therefore, the design of small molecule fluorescent sensors targeting NTR is of great significance for the detection and diagnosis of plant pathogenic bacteria. In this study, a new fluorescent sensor targeting NTR was discovered and then successfully applied to the imaging of zebrafish and pathogenic bacteria. Most importantly, the developed sensor achieved the real-time diagnosis of Brassica napus L. infected with bacteria, which provides a promising tool for examining the temporal and spatial infection of plant pathogens in precision agriculture.
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