期刊
ANALYTICAL CHEMISTRY
卷 94, 期 46, 页码 16246-16253出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.2c04101
关键词
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资金
- National Natural Science Foundation of China [22172040, 21974031]
- Department of Science and Techniques of Guangdong Province [2021A1515010180, 2019B010933001]
- Department of Guangdong Provincial Public Security [GZQC20-PZ11-FD084]
- Science and Technology Projects in Guangzhou [202201000002]
- Guangzhou Municipal Science and Technology Bureau [202102010449]
- Innovation Team Scientific Research Project of Guangzhou Education Bureau [202235344]
In this study, a near-infrared light-driven NaYF4:Yb/Er-TiO2-Ti3C2 heterostructure-based photo-electrochemical biosensing platform was developed for highly sensitive detection of D-serine. The platform exhibited excellent performance with a wide linear range and low detection limit, and showed high detection reproducibility and accuracy in human serum and rat cerebrospinal fluid samples. This platform may provide new opportunities for detecting other disease-related biomarkers.
A near-infrared (NIR) light-driven NaYF4:Yb/Er- TiO2-Ti3C2 (NYF-TiO2-Ti3C2) heterostructure-based photo -electrochemical (PEC) biosensing platform was constructed for highly sensitive D-serine (D-ser) detection. Accurate D-ser detection depends on the model biocatalyst, D-amino acid oxidase (DAAO), which converts D-ser into hydroxypyruvate and an equimolar concentration of hydrogen peroxide (H2O2) via an enzymatic reaction. The TiO2-Ti3C2 semiconductor and NaYF4:Yb/Er optical transducer formed a Schottky junction that provided an irreversible channel for electron transfer. Infrared light was converted into absorbable multiemission light, thereby effectively increasing light absorption. Simultaneously, the generated H2O2 rapidly scavenged photogenerated holes to separate electron-hole pairs, which amplified the photocurrent signal. Under optimal conditions, the NIR light-driven PEC biosensor exhibited an excellent PEC performance for D-ser detection, with a wide linear range of 2-1650 mu mol L-1 and detection limit as low as 0.286 mu mol L-1. Importantly, high detection reproducibility and accuracy were achieved using this strategy for analyzing human serum and rat cerebrospinal fluid (CSF) specimens. The admirable applicability of the NYF-TiO2-Ti3C2-based PEC biosensor for detecting D-ser may lead to further opportunities for detecting other disease-related biomarkers.
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