Sb-doped FeOCl nanozyme-based biosensor for highly sensitive colorimetric detection of glutathione

Nanozymes have been emerging as substitutes for natural enzymes to construct biosensors towards biomolecular detection. However, the detection of glutathione (GSH) by nanozyme-based biosensors still remains a great challenge for research on catalytic activity enhancement and the detection mechanism. In this work, Sb-doped iron oxychloride (Sb-FeOCl) with a well-defined nanorod-like structure is prepared by high-temperature calcination. Sb-FeOCl nanorods have high peroxidase-like activity, which can catalyze the decomposition of H2O2 into Greek ano teleiaOH and then oxidize 3,3 ',5,5 '-tetramethylbenzidine (TMB). In view of these intriguing observations, a reliable colorimetric method with a simple mixing and detection strategy is developed for the detection of GSH. The linear range of GSH detection is 1-36 mu M. The detection limit of GSH reaches a low level of 0.495 mu M (3 sigma/slope). The GSH sensing system also exhibits excellent specificity and anti-interference. Taking advantage of the advantages of the Sb-FeOCl nanorod-based biosensor, it can be used to quantitatively detect GSH levels in human serum. It can be anticipated that the Sb-FeOCl nanorods have broad prospects in the field of enzymatic biochemical reactions.

Automated summary

This work investigated the catalytic activity of Sb-doped iron oxychloride (Sb-FeOCl) nanorods and discovered their high peroxidase-like activity, which could be used for the detection of glutathione (GSH). A reliable colorimetric method for GSH detection was developed, with a linear detection range of 1-36 μM and a detection limit of 0.495 μM. The Sb-FeOCl nanorods exhibited excellent specificity and anti-interference, making them suitable for quantitative detection of GSH levels in human serum. This study suggests that Sb-FeOCl nanorods have promising applications in enzymatic biochemical reactions.