4.7 Article

Coupling of nitric acid digestion and anion-exchange resin separation for the determination of methylmercury isotopic composition within organisms

期刊

ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 415, 期 5, 页码 759-774

出版社

SPRINGER HEIDELBERG
DOI: 10.1007/s00216-022-04468-8

关键词

Mercury isotopes; Methylmercury; Anion-exchange resin; Nitric acid digestion; Aquatic organisms; Separation

向作者/读者索取更多资源

Isotope ratios of methylmercury (MeHg) within organisms can be used to identify sources of MeHg that have accumulated in food webs. A new method for extracting and isolating MeHg from fish and aquatic invertebrates has been developed for compound-specific isotopic analysis. This method provides a streamlined and reliable technique that produces reproducible results.
Isotope ratios of methylmercury (MeHg) within organisms can be used to identify sources of MeHg that have accumulated in food webs, but these isotopic compositions are masked in organisms at lower trophic levels by the presence of inorganic mercury (iHg). To facilitate measurement of MeHg isotope ratios in organisms, we developed a method of extracting and isolating MeHg from fish and aquatic invertebrates for compound-specific isotopic analysis involving nitric acid digestion, batch anion-exchange resin separation, and pre-concentration by purge and trap. Recovery of MeHg was quantified after each step in the procedure, and the average cumulative recovery of MeHg was 93.4 & PLUSMN; 2.9% (1 SD, n = 28) for biological reference materials and natural biota samples and 96.9 & PLUSMN; 1.8% (1 SD, n = 5) for aqueous MeHgCl standards. The amount of iHg impurities was also quantified after each step, and the average MeHg purity was 97.8 & PLUSMN; 4.3% (1 SD, n = 28) across all reference materials and natural biota samples after the final separation step. Measured MeHg isotopic compositions of reference materials agreed with literature values obtained using other MeHg separation techniques, and MeHg isotope ratios of aqueous standards, reference materials, and natural biota samples were reproducible. On average, the reproducibility associated with reference material process replicates (2 SD) was 0.10 & PTSTHOUSND; for delta(MeHg)-Me-202 and 0.04 & PTSTHOUSND; for & UDelta;(MeHg)-Me-199. This new method provides a streamlined, reliable technique that utilizes a single sample aliquot for MeHg concentration and isotopic analysis. This promotes a tight coupling between MeHg concentration, %MeHg, and Hg isotopic composition, which may be especially beneficial for studying complex food webs with multiple isotopically distinct sources of iHg and/or MeHg.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据