期刊
ANALYTICA CHIMICA ACTA
卷 1238, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aca.2022.340639
关键词
Single-walled carbon nanotubes; RNA protection; RNA extraction; SARS-CoV-2; RT-qPCR
The false-negative result of nucleic acid testing is one of the important causes of COVID-19 transmission. This study developed a method using single-walled carbon nanotubes (SWCNTs) to protect and extract SARS-CoV-2 RNA, improving the sensitivity of RT-qPCR detection by 8-32 fold. The protection of RNA by SWCNTs allows for increased RNA load in downstream assays.
The false-negative result of nucleic acid testing is an important cause of continued spread of COVID-19, while SARS-CoV-2 RNA degradation during transportation and nucleic acid extraction can lead to false-negative re-sults. Here, we investigated that single-walled carbon nanotubes (SCNTs) could protect RNA from degradation for at least 4 days at room temperature. By constructing magnetism-functionalized SCNTs (MSCNTs), we developed a method that enabled protection and simple extraction of SARS-CoV-2 RNA, and the RNA-bound MSCNTs can be directly used for reverse transcription polymerase chain reaction (RT-qPCR) detection. The experimental results showed that 1 mu g of MSCNTs adsorbed up to 24 ng of RNA. Notably, the MSCNTs-based method for extracting SARS-CoV-2 RNA from simulated nasopharyngeal swabs and saliva samples with mean recovery rates of 103% and 106% improved the sensitivity of RT-qPCR detection by 8-32 fold in comparison to current common methods. This improvement was largely attributable to the protection of RNA, enabling increased RNA load for downstream assays.
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