Subtype Distribution and Drug Resistance Patterns Among HIV-1 Strains Prevalent in Makassar, Indonesia

Human immunodeficiency virus type 1 (HIV-1) is characterized by a large degree of genetic variability because of high rates of recombination and mutation, sizable population sizes, and rapid replication. Therefore, this study investigated HIV-1 subtype distribution and the appearance of drug resistance mutations (DRMs) in viruses that are prevalent in Makassar, South Sulawesi, Indonesia. The HIV-1 pol, env, and gag genes were amplified from 63 infected individuals and sequenced for a subtyping analysis. CRF01_AE was identified as the predominant HIV-1 circulating recombinant form (CRF) in Makassar, South Sulawesi, Indonesia. Subtype B and recombinant viruses containing CRF01_AE, CRF02_AG, and/or subtype B gene fragments were also detected. Several major DRMs against non-nucleoside reverse transcriptase inhibitors were found among antiretroviral therapy (ART)-experienced subjects, whereas ART-naive subjects did not possess any transmitted drug resistance. The prevalence of DRMs was very high among ART-experienced subjects; therefore, further surveillance is required in this region.

Automated summary

This study investigated the distribution of HIV-1 subtypes and the emergence of drug resistance mutations (DRMs) in Makassar, South Sulawesi, Indonesia. CRF01_AE was identified as the predominant circulating recombinant form (CRF) in this region, along with subtype B and recombinant viruses containing CRF02_AG and/or subtype B gene fragments. DRMs against non-nucleoside reverse transcriptase inhibitors were found among ART-experienced individuals, while no transmitted drug resistance was observed in ART-naive individuals.