期刊
JOURNAL OF NEUROIMMUNOLOGY
卷 290, 期 -, 页码 80-83出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jneuroim.2015.11.009
关键词
Paraneoplastic; CV2 (CRMP5) antibodies; Cancer; Immunoblot
资金
- Fondo de Investigaciones Sanitarias, Madrid, Spain [PI12/00,611]
- ICREA Funding Source: Custom
CV2 antibodies (CV2-ab) associate with paraneoplastic neurological syndromes (PNS) and small-cell lung cancer. This study was designed to assess the sensitivity of two widely used anti-CV2 commercial kits. Fifty three sera with CV2-ab identified by immunohistochemistry on paraformaldehyde-perfused rat brain were tested with two commercial immunoblot kits (Euroimmun AG, and Ravo Diagnostika) and 4 (7.5%) of them were negative with the commercial kits. The 4 samples were positive by immunofluorescence on HEK293 cells transfected with CRMP5 and immunoblot of these cells lysate. A few CV2-ab-positive sera may be missed by commercial immunoblots. Negative samples from patients with high suspicion for PNS should be tested by alternative methods. (C) 2015 Elsevier B.V. All rights reserved.
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