Bioengineered 3D Skeletal Muscle Model Reveals Complement 4b as a Cell-Autonomous Mechanism of Impaired Regeneration with Aging

A mechanistic understanding of cell-autonomous skeletal muscle changes after injury can lead to novel interventions to improve functional recovery in an aged population. However, major knowledge gaps persist owing to limitations of traditional biological aging models. 2D cell culture represents an artificial environment, while aging mammalian models are contaminated by influences from non-muscle cells and other organs. Here, a 3D muscle aging system is created to overcome the limitations of these traditional platforms. It is shown that old muscle constructs (OMC) manifest a sarcopenic phenotype, as evidenced by hypotrophic myotubes, reduced contractile function, and decreased regenerative capacity compared to young muscle constructs. OMC also phenocopy the regenerative responses of aged muscle to two interventions, pharmacological and biological. Interrogation of muscle cell-specific mechanisms that contribute to impaired regeneration over time further reveals that an aging-induced increase of complement component 4b (C4b) delays muscle progenitor cell amplification and impairs functional recovery. However, administration of complement factor I, a C4b inactivator, improves muscle regeneration in vitro and in vivo, indicating that C4b inhibition may be a novel approach to enhance aged muscle repair. Collectively, the model herein exhibits capabilities to study cell-autonomous changes in skeletal muscle during aging, regeneration, and intervention.

Automated summary

Understanding the cell-autonomous changes in skeletal muscle during aging, regeneration, and intervention is crucial for improving functional recovery in the elderly. However, traditional biological aging models have limitations. To overcome these limitations, a 3D muscle aging system was created and it was found that old muscle constructs (OMC) exhibit a sarcopenic phenotype and impaired regenerative capacity. Enhanced expression of complement component 4b (C4b) in aging muscle was identified as a contributing factor to impaired muscle regeneration, but inhibition of C4b improved regeneration in vitro and in vivo, suggesting a novel approach to enhance aged muscle repair.