4.8 Review

Toward Rapid and Accurate Molecular Diagnostics at Home

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Diagnostics for COVID-19: moving from pandemic response to control

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Summary: Diagnostics play a crucial role in the response to the COVID-19 pandemic. Molecular tests like PCR are recommended for confirming diagnosis in symptomatic individuals, while antigen rapid detection tests have the advantage of being easier to perform with faster results and lower cost. Antibody tests can inform public policy but should not be used as proof of immunity. All three types of tests continue to be important in transitioning from pandemic response to control.

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SARS-CoV-2 RNAemia Predicts Clinical Deterioration and Extrapulmonary Complications from COVID-19

Nikhil Ram-Mohan et al.

Summary: Measurement of SARS-CoV-2 RNA from the plasma of COVID-19 patients using digital polymerase chain reaction can predict disease severity, clinical deterioration, and extrapulmonary complications. It may guide patient triage and management effectively.

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Fabrication of Wearable PDMS Device for Rapid Detection of Nucleic Acids via Recombinase Polymerase Amplification Operated by Human Body Heat

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Summary: In this study, a wearable nucleic acid amplification microdevice was developed, which utilizes human body heat for reaction and provides an instrument-free solution for DNA amplification in resource-limited settings. The device is flexible, adhesive, and capable of rapid amplification.

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Rapid SARS-CoV-2 Detection Using the Lucira™ Check It COVID-19 Test Kit

Maya Zahavi et al.

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Considerations for diagnostic COVID-19 tests

Olivier Vandenberg et al.

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Rolling Circle Amplification in Integrated Microsystems: An Uncut Gem toward Massively Multiplexed Pathogen Diagnostics and Genotyping

Ruben R. G. Soares et al.

Summary: The text highlights the importance of developing robust methods for detecting specific nucleic acid sequences, with a focus on pathogen diagnostics. It introduces the underexplored technology of padlock probes coupled with rolling circle amplification (PLP-RCA) in the arena of isothermal nucleic acid amplification tests (NAATs), emphasizing its unprecedented degree of multiplexing, specificity, versatility, and potential for integration in miniaturized point-of-care platforms. The text also discusses key developments in miniaturization and integration of PLP-RCA to enhance sensitivity, maximize throughput, and streamline analytical workflow for rapid pathogen diagnostics.

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Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy

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Clinical Performance of the Point-of-Care cobas Liat for Detection of SARS-CoV-2 in 20 Minutes: a Multicenter Study

Glen Hansen et al.

Summary: The study compared the clinical performance of a 20-minute point-of-care RT-PCR test for SARS-CoV-2 with the widely used laboratory test, showing a high level of agreement between the two methods. Rapid RT-PCR testing at point of care enables timely infection control and individual care decisions for COVID-19.

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Exosome detection via the ultrafast-isolation system: EXODUS

Yuchao Chen et al.

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Test sensitivity is secondary to frequency and turnaround time for COVID-19 screening

Daniel B. Larremore et al.

Summary: The COVID-19 pandemic has led to a public health crisis due to the spread of SARS-CoV-2 through presymptomatic, symptomatic, and asymptomatic individuals. Effective population screening for the virus is essential for reopening societies and controlling its spread, with testing frequency and reporting speed playing crucial roles in its success. Test sensitivity is only marginally important compared to these factors.

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Article Infectious Diseases

Evaluation of the BioFire® COVID-19 test and Respiratory Panel 2.1 for rapid identification of SARS-CoV-2 in nasopharyngeal swab samples

Eric J. Eckbo et al.

Summary: The BioFire COVID-19 Test and RP2.1 are rapid and highly sensitive assays for detecting SARS-CoV-2, which have received emergency use authorization from the FDA and Health Canada. Both tests demonstrated 100% concordance with a laboratory-developed test for clinical samples, showing their effectiveness for rapid testing in clinical laboratories.

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Qingfu Zhu et al.

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Bhavesh D. Kevadiya et al.

Summary: The outbreak of SARS-CoV-2 causing COVID-19 has led to diverse and severe symptoms, making accurate diagnosis a key challenge. Rapid diagnosis and treatment measures are crucial in controlling the spread of the virus. The development of diagnostic tools, including nanomaterial-based ones, is essential in the fight against the pandemic.

NATURE MATERIALS (2021)

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Harmony COVID-19: A ready-to-use kit, low-cost detector, and smartphone app for point-of-care SARS-CoV-2 RNA detection

Nuttada Panpradist et al.

Summary: The Harmony COVID-19 test offers a cost-effective and user-friendly solution for detecting SARS-CoV-2 infection, with a high success rate and accuracy, as demonstrated through clinical evaluations and usability testing.

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Woven Electroanalytical Biosensor for Nucleic Acid Amplification Tests

Shirin Khaliliazar et al.

Summary: Fiber-based biosensors with microfluidic structures and electroanalytical readout are used for Nucleic Acid Amplification Testing. Two types of fiber-based electrodes are characterized, and a woven electrochemical DNA sensor is fabricated for specific detection of genomic DNA of Staphylococcus epidermidis.

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CRISPR/Cas12a-Based Versatile Method for Checking Quantitative Polymerase Chain Reaction Samples with Cycles of Threshold Values in the Gray Zone

Yanju Chen et al.

Summary: qPCR gray zone testing results in uncertainty, while CRISPR technology can enhance detection signal sensitivity. A CRISPR-based checking method is proposed to solve gray zone problems, effectively eliminating uncertainty in sample classification without increasing workload in PCR detection.

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Lab-on-a-Disc for Point-of-Care Infection Diagnostics

Vijaya Sunkara et al.

Summary: In response to the COVID-19 pandemic, reliable, inexpensive, and rapid diagnostic tools are crucial. Recent advances in centrifugal microfluidic devices show promise in delivering fully automated POC infection diagnostics, allowing for easy sample collection, testing, and result output with high performance and accuracy.

ACCOUNTS OF CHEMICAL RESEARCH (2021)

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A lab-on-a-chip platform for integrated extraction and detection of SARS-CoV-2 RNA in resource-limited settings

Pablo Rodriguez-Mateos et al.

Summary: Efforts are needed to develop rapid and accurate diagnostic tools for extensive testing of COVID-19. Current gold standard nucleic acid tests require trained personnel and specialist instrumentation, which hampers turnaround time and test accessibility, especially in low-resource settings.

ANALYTICA CHIMICA ACTA (2021)

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Wearable materials with embedded synthetic biology sensors for biomolecule detection

Peter Q. Nguyen et al.

Summary: This study demonstrates the development of wearable devices functionalized with freeze-dried, cell-free synthetic circuits for noninvasive detection of biomolecules, including virus nucleic acids, without the need for living engineered bacteria. The technology shows promise for expanding opportunities in physiological status monitoring and disease detection.

NATURE BIOTECHNOLOGY (2021)

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Oil immersed lossless total analysis system for integrated RNA extraction and detection of SARS-CoV-2

Duane S. Juang et al.

Summary: The authors report the development of OIL-TAS, a system that integrates RNA extraction and detection into a single device, which shows promising performance in clinical sample testing with the potential to offer a faster, cheaper, and easier alternative for infectious disease testing.

NATURE COMMUNICATIONS (2021)

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Jacquelyn Jones et al.

Summary: The collection methods of fecal samples have significant impact on microbiome studies, necessitating sample homogenization for improved data quality; findings indicate a shifting trend in microbial and metabolite composition over short periods of time; the recommended method for fecal sample collection involves collecting the first full bowel movement of the day and freezing the sample immediately.

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Multifunctional Chemical Sensing Platform Based on Dual-Resonant Infrared Plasmonic Perfect Absorber for On-Chip Detection of Poly(ethyl cyanoacrylate)

Dongxiao Li et al.

Summary: A multifunctional chemical sensing platform based on dual-resonant SEIRA device is reported for sensitive and multifunctional on-chip detection of PECA. The sensor offers dual-band fingerprint vibration identification, sub-nm level detection limit, and ultrahigh sensitivity in thickness measurement, showing potential for on-chip chemical sensing.

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Qun Li et al.

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Rapid isothermal amplification and portable detection system for SARS-CoV-2

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