期刊
ACTA PARASITOLOGICA
卷 68, 期 1, 页码 130-144出版社
SPRINGER INT PUBL AG
DOI: 10.1007/s11686-022-00640-3
关键词
Trypanosoma congolense; Drug resistance; Isometamidium; Diminazene; Homidium; Kenya
The study aimed to detect drug-resistant Trypanosoma congolense in Lambwe, Kenya and assess the reliability of a molecular test backed with mice tests in detecting drug sensitivity. The results showed the existence of T. congolense associated with homidium and diminazene resistance in Lambwe. Polymorphisms were found in Lambwe strains and the TcoAde2 gene was found to be unrelated to drug sensitivity. The study concluded that the combination of molecular and mice tests is a reliable method for testing drug sensitivity.
Purpose African animal trypanosomiasis (AAT) is a disease affecting livestock in sub-Saharan Africa. The use of trypanocidal agents is common practice to control AAT. This study aimed to identify drug-resistant Trypanosoma congolense in Lambwe, Kenya, and assess if molecular test backed with mice tests is reliable in detecting drug sensitivity. Methods Blood samples were collected from cattle, in Lambwe, subjected to buffy coat extraction and Trypanosoma spp. detected under a microscope. Field and archived isolates were subjected to molecular characterization. Species-specific T. congolense and TcoAde2 genes were amplified using PCR to detect polymorphisms. Phylogenetic analysis were performed. Four T. congolense isolates were evaluated individually in 24 test mice per isolate. Test mice were then grouped (n=6) per treatement with diminazene, homidium, isometamidium, and controls. Mice were subsequently assessed for packed cell volume (PCV) and relapses using microscopy. Results Of 454 samples, microscopy detected 11 T. congolense spp, eight had TcoAde2 gene, six showed polymorphisms in molecular assay. Phylogenetic analysis grouped isolates into five. Two archived isolates were homidium resistant, one was also diminazene resistant in mice. Two additional isolates were sensitive to all the drugs. Interestingly, one sensitive isolate lacked polymorphisms, while the second lacked TcoAde2, indicating the gene is not involved in drug sensitivity. Decline in PCV was pronounced in relapsed isolates. Conclusion T. congolense associated with homidium and diminazene resistance exist in Lambwe. The impact can be their spread and AAT increase. Polymorphisms are present in Lambwe strains. TcoAde2 is unlikely involved in drug sensitivity. Molecular combined with mice tests is reliable drug sensitivity test and can be applied to other genes. Decline in PCV in infected-treated host could suggest drug resistance.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据