Yeast Display Enables Identification of Covalent Single-Domain Antibodies against Botulinum Neurotoxin Light Chain A

Review Biochemistry & Molecular Biology

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Summary: By incorporating latent bioreactive amino acids into proteins through genetic code expansion, selective covalent linkages can be generated, enabling the development of covalent protein drugs with improved therapeutic potential.

CURRENT OPINION IN CHEMICAL BIOLOGY (2022)

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Probing the structure and function of the protease domain of botulinum neurotoxins using single-domain antibodies

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Longxing Cao et al.

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NATURE (2022)

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ACS SYNTHETIC BIOLOGY (2022)

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ACS SYNTHETIC BIOLOGY (2022)

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Mariha Islam et al.

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ACS CHEMICAL BIOLOGY (2021)

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Shiyu Chen et al.

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NATURE BIOTECHNOLOGY (2021)

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Christopher J. Podracky et al.

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NATURE CHEMICAL BIOLOGY (2021)

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Broad and potent activity against SARS-like viruses by an engineered human monoclonal antibody

C. Garrett Rappazzo et al.

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Neuronal delivery of antibodies has therapeutic effects in animal models of botulism

Patrick M. McNutt et al.

Summary: Research has shown that using atoxic derivatives of botulinum neurotoxin can effectively deliver function-blocking antibodies to the neuronal cytosol, neutralizing the toxicity of botulinum toxin. This method has been successful in animal experiments and may enable the treatment of other previously inaccessible intracellular targets.

SCIENCE TRANSLATIONAL MEDICINE (2021)

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Delivery of single-domain antibodies into neurons using a chimeric toxin-based platform is therapeutic in mouse models of botulism

Shin-Ichiro Miyashita et al.

Summary: Efficient intracellular delivery to neurons was achieved by fusing a chimeric toxin-based platform targeting BoNTs with nanobodies, effectively neutralizing BoNT/A and BoNT/B in mice. This post-exposure treatment approach shortened paralysis duration and rescued mice from lethal doses of BoNT/A toxicity, providing a promising strategy for botulism therapy.

SCIENCE TRANSLATIONAL MEDICINE (2021)

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Heng Zhang et al.

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Yaping Cheng et al.

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BIOORGANIC & MEDICINAL CHEMISTRY (2021)

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Elma Mons et al.

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JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2021)

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Hao Chen et al.

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CURRENT OPINION IN MICROBIOLOGY (2021)

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A Genetically Encoded Fluorosulfonyloxybenzoyl-L-lysine for Expansive Covalent Bonding of Proteins via SuFEx Chemistry

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Yeast Display Enables Identification of Covalent Single-Domain Antibodies against Botulinum Neurotoxin Light Chain A

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