期刊
ACS APPLIED MATERIALS & INTERFACES
卷 15, 期 3, 页码 4652-4667出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.2c16584
关键词
tendon; topology; three-dimensional; stem cell; regeneration
This study used an ordered 3D sandwich model to investigate the cell response in tendons and found that tendon stem/progenitor cells (TSPCs) maintained their ordering growth in the 3D model. The 3D-aligned TSPCs exhibited enhanced tenogenic differentiation and a less inflammatory phenotype compared to the 2D model, which can promote tendon regeneration.
Tendon injuries are some of the most commonly diagnosed musculoskeletal diseases. Tendon regeneration is sensitive to the topology of the substitute as it affects the cellular microenvironment and homeostasis. To bionic in vivo three-dimensional (3D) aligned microenvironment, an ordered 3D sandwich model was used to investigate the cell response in the tendon. First, high-resolution 3D printing provided parallel-grooved topographical cues on the hydrogel surface. Then the cells were seeded on its surface to acquire a 2D model. Afterward, an additional hydrogel coating layer was applied to the cells to create the 3D model. The interaction between cells and order structures in three-dimensions is yet to be explored. The study found that the tendon stem/progenitor cells (TSPCs) still maintain their ordering growth in the 3D model as in the 2D model. The study also found that the 3D-aligned TSPCs exhibited enhanced tenogenic differentiation through the PI3K-AKT signaling pathway and presented a less inflammatory phenotype than those in the 2D model. The in vivo implantation of such a 3D-aligned TSPC composite promoted tendon regeneration and mitigated heterotopic ossification in an Achilles defect model. These findings demonstrated that 3D-aligned TSPCs within a biomimetic topology environment are promising for functional tendon regeneration.
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