期刊
JOURNAL OF NEUROCHEMISTRY
卷 137, 期 2, 页码 216-225出版社
WILEY-BLACKWELL
DOI: 10.1111/jnc.13525
关键词
addiction; Amygdala; conditioned place preference; extinction; methamphetamine; mGluR5
资金
- National Health Research Institute [NHRI-EX101-10117NI]
- National Science Council [MOST 103-2321-B-006-008]
- Aim for the Top University Project of the National Cheng-Kung University
Addiction is thought to be a memory process between perception and environmental cues and addicted patients often relapse when they come into contact with the drug-related context once again. Here, we used a conditioned place preference protocol to seek a more effective extinction methodology of methamphetamine (METH) memory and delineate its underlying mechanism. Conditioning METH for 3days in mice markedly increased the time spent in the METH-paired compartment. Then the mice were conditioned with saline for 6days, from day 6 to day 11, a procedure termed extinction training. However, METH memory returned after a priming injection of METH. We prolonged extinction duration from 6 to 10days and found that this extensive extinction (EE) training prevented priming effect. At the molecular level, we discovered that prolonged extinction training reversed the METH-conditioned place preference-induced increase in surface expression of GluA2 and alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA)/NMDA ratio in the basolateral amygdala. In addition, we found that extinction with metabotropic glutamate receptor 5 (mGluR5) activation had similar results to EE: reduced relapse after extinction, decreased synaptic AMPA receptors AMPARs and the AMPA/NMDA ratio. On the contrary, EE with mGluR5 inhibition suppressed the results of EE. These data indicate that EE training-elicited inhibition of METH-primed reinstatement is mediated by the mGluR5.
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