Emestrins: Anti-Cryptococcus Epipolythiodioxopiperazines from Podospora australis

Eleven emestrin-type epipolythiodioxopiperazines, including four new compounds, emestrins H-K (1-4); were isolated from the crude extracts of two strains of the coprophilous fungus Podospora australis. The structures of 1-4 were established primarily by analysis of NMR data, and the absolute configuration of C-6 in 1 was independently assigned using the modified Mosher method. Four of the known emestrins obtained (emestrins C-E and MPC1001C) were found to selectively inhibit the growth of Cryptococcus neoformans. These results also represent the first report of chemistry from any strain of P. australis.