期刊
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
卷 16, 期 3, 页码 2488-2491出版社
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jnn.2016.11071
关键词
Magnetic Particles; Silica Nanospheres; QDs; FRET; K-Ras
类别
资金
- Human Resources Development program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant - Korea government Ministry of Trade, Industry and Energy [20124030200010]
- National Research Foundation - Korean government (MEST) [NRF-2006-2005382]
Silica nanospheres containing magnetic particles were prepared, and CdSe/ZnS QDs functionalized with carboxyl group were incorporated into the silica nanospheres by EDC/NHS coupling reaction. The silica nanospheres were prepared by a co-precipitation of ferrous and ferric solutions followed by the sol-gel reaction of TEOS (tetraethoxysilane) and APTES (3-aminopropyltriethoxysilane) using base catalyst. The size of magnetic silica nanospheres was confirmed by Transmission electron microscope (TEM). Thiol group modified single stranded oligonucleotides were immobilized on the surface of QDs and fluorescence quenching by intercalation dye (TOTO-3) after hybridization with target oligonucleotide was observed. The fluorescence from QDs could be quenched by intercalating dye (TOTO-3) after hybridization of target DNA to probe DNA. This shows that the magnetic silica-QD-DNA probe can be used to detect specific DNA.
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