期刊
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
卷 123, 期 -, 页码 53-61出版社
ELSEVIER
DOI: 10.1016/j.molcatb.2015.11.009
关键词
TG2; Enzyme kinetics; LC-MS; Biocatalysis; Molecular modeling
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Canada Foundation for Innovation
- Ontario Research Fund
- FRQNT
- Ontario Graduate Scholarship
Biocatalytic methods for peptide synthesis are of high value due to the rapidly increasing approval of peptide-based therapeutics and the need to develop new analogs. Guinea pig liver transglutaminase (gTG2) catalyzes the cross-linking of peptides and proteins via the formation of gamma-glutamyl-is an element of-lysyl isopeptide bonds. In this study, we investigate gTG2-catalyzed peptide bond formation between various amino acid-derived donor and acceptor substrates. Using LC-MS analysis, we demonstrate that gTG2 forms GIy-Xaa and D-Ala-Gly dipeptide products, confirming that its natural transamidation activity can be co-opted for peptide synthesis. An aromatic ester of Gly was the most efficient acyl-donor substrate tested; aromatic esters of D-Ala and L-Ala showed 50-fold lower reactivity or no reactivity, respectively. A computational strategy combining computational protein design algorithms and molecular dynamics simulations was developed to model the binding modes of donor substrates in the gTG2 active site. We show that the inability of gTG2 to efficiently catalyze peptide synthesis from donors containing alanine results from the narrow substrate binding tunnel, which prevents bulkier donors from adopting a catalytically productive binding mode. Our observations pave the way to future protein engineering efforts to expand the substrate scope of gTG2 in peptide synthesis, which may lead to useful biocatalysts for the synthesis of desirable bioactive molecules. (C) 2015 Elsevier B.V. All rights reserved.
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