Cashew apple bagasse as a support for the immobilization of lipase B from Candida antarctica: Application to the chemoenzymatic production of (R)-Indanol

Lipase B from Candida antarctica lipase expressed in Aspergillus niger has been covalently immobilized on cashew apple bagasse, and it stability and performance in the chemoenzymatic synthesis of (R)-Indanol has been evaluated. The cashew apple bagasse (CAB) has been treated with alkaline hydrogen peroxide and activated with glycidol (GLY) to obtain glyoxyl groups, in addition to ethylenediamine and further activation with glutaraldehyde (GEG).The maximum loading of the support was 10 mg proteinig support, and its thermal and solvent stability was much higher than that of the soluble enzyme. The CAB-GEG preparation (with 95% yield of immobilization and 124% activity recovery) was used in the hydrolysis of ethyl rac-indanyl acetate using an enzyme/substrate ratio of 2: 1 and a pH value of 7.0 at 30 degrees C for 24 h. The conversion attained was 50% of conversion and e.e. of 99%. The reusability studies showed maintenance of conversion and e.e. during five cycles. The results suggest that the new CAB support may be an excellent alternative for lipase immobilization and stabilization. (C) 2016 Elsevier B.V. All rights reserved.