期刊
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
卷 96, 期 -, 页码 11-25出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.yjmcc.2015.02.006
关键词
Cross-bridge cycle; Viscoelasticity; Cardiac muscle; Force generation; Metabolites; Sinusoidal perturbation analysis
资金
- National Institutes of Health [HL072011, GM094503, HL59408]
Despite extensive study over the past six decades the coupling of chemical reaction and mechanical processes in muscle dynamics is not well understood. We lack a theoretical description of how chemical processes (metabolite binding, ATP hydrolysis) influence and are influenced by mechanical processes (deformation and force generation). To address this need, a mathematical model of the muscle cross-bridge (XB) cycle based on Huxley's sliding filament theory is developed that explicitly accounts for the chemical transformation events and the influence of strain on state transitions. The model is identified based on elastic and viscous moduli data from mouse and rat myocardial strips over a range of perturbation frequencies, and MgATP and inorganic phosphate (Pi) concentrations. Simulations of the identified model reproduce the observed effects of MgATP and MgADP on the rate of force development. Furthermore, simulations reveal that the rate of force re-development measured in slack-restretch experiments is not directly proportional to the rate of XB cycling. For these experiments, the model predicts that the observed increase in the rate of force generation with increased Pi concentration is due to inhibition of cycle turnover by Pi. Finally, the model captures the observed phenomena of force yielding suggesting that it is a result of rapid detachment of stretched attached myosin heads. (C) 2015 Elsevier Ltd. All rights reserved.
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