4.5 Article

Very low likelihood that cultivated oysters are a vehicle for SARS-CoV-2: 2021-2022 seasonal survey at supermarkets in Kyoto, Japan

期刊

HELIYON
卷 8, 期 10, 页码 -

出版社

ELSEVIER SCI LTD
DOI: 10.1016/j.heliyon.2022.e10864

关键词

SARS-CoV-2; Cultivated oysters; Quantitative RT-PCR; Survey

资金

  1. Ministry of Education, Culture, Sports, Science and Technology (MEXT)
  2. Japan Society for the Promotion of Science-JSPS KAKENHI [JP21H03180]

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The study investigated the presence of SARS-CoV-2 in cultivated oysters sold in Japan and evaluated the risk of food-borne transmission. The results indicated that consumption of oysters cultivated in coastal areas may not be a risk factor for SARS-CoV-2 outbreaks, suggesting that well-developed sewage treatment facilities contribute to preventing viral contamination.
The pandemic caused by novel coronavirus disease of 2019 (COVID-19) is a global threat. Wastewater surveillance in Japan and abroad has led to the detection of SARS-CoV-2, causing concern that SARS-CoV-2 in the feces of infected persons may contaminate the aquatic environment. Bivalves such as oysters cultivated in coastal areas are known to filter and concentrate viruses such as norovirus present in seawater in their bodies; however, whether they do so with SARS-CoV-2 is unknown. Therefore, we examined cultivated oysters sold in Japan for the presence of SARS-CoV-2 between October 2021 and April 2022 to clarify the extent of viral contamination and evaluate the risk of food-borne transmission of SARS-CoV-2. Porcine epidemic diarrhea virus (PEDV), known as pig coronavirus, was used to spike midgut-gland samples as a whole process control. The presence of SARS-CoV-2 and PEDV was investigated using a modified polyethylene glycol precipitation method and RT-qPCR. While all samples spiked with the whole process control were positive, no SARS-CoV-2 was detected in any of the 145 raw oyster samples surveyed, despite a marked increase in infections caused by the Omicron variant from January to April 2022 in Japan. Therefore, our results suggest that with well-developed sewage treatment facilities, consumption of oysters cultivated in coastal areas may not be a risk factor for SARS-CoV-2 outbreaks.

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