期刊
FOOD CHEMISTRY-X
卷 15, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.fochx.2022.100446
关键词
Sweet taste receptor; Time -intensity; Cyclamate; NHDC; Aspartame; Acesulfame K
资金
- Austrian National Foundation for Research, Technology, and Development
- Symrise AG
- Austrian Federal Ministry for Digital and Economic Affairs
This study investigated the impact of different binding sites of agonists and antagonists on the temporal sensory properties of sweet tasting compounds. The results suggest that the binding site does not have a major effect on the time-intensity profile of the tested sweeteners.
Currently, there is limited insight into the influence of the different binding sites of agonists and antagonists of the sweet taste receptor TAS1R2/TAS1R3 on temporal sensory properties of sweet tasting compounds. We investigated whether the binding site and a competitive or allosteric inhibition of TAS1R2/TAS1R3 influence the time-dependent sensory perception and in vitro TAS1R2/TAS1R3-activation profiles. We compared time -intensity ratings of cyclamate, NHDC, acesulfame K, and aspartame with and without lactisole with the corresponding TAS1R2/TAS1R3-activation in transfected HEK293 cells. In combination with lactisole, cyclamate and NHDC demonstrated a shift of the dose-response curve corresponding to a competitive inhibition by lactisole in the sensory and the cell experiments. Allosteric inhibition by lactisole for aspartame and acesulfame K was seen in the cell experiments, but not the sensory ratings. In conclusion, the data do not support a major impact of the binding site on the time-intensity profile of the tested sweeteners.
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