Simultaneous Detection of Chlorzoxazone and Paracetamol Using a Greener Reverse-Phase HPTLC-UV Method

In the literature, greener/eco-friendly analytical techniques for simultaneous estimation of chlorzoxazone (CZN) and paracetamol (PCT) are scarce. As a consequence, greener reverse-phase high-performance thin-layer chromatography with ultraviolet (HPTLC-UV) detection was developed and validated for simultaneous estimation of CZN and PCT in commercial capsules and tablets. The greenness of the proposed HPTLC-UV technique was assessed quantitatively by utilizing the Analytical GREENness (AGREE) methodology. For simultaneous estimation of CZN and PCT, the greener HPTLC-UV technique was linear in the 40-1600 ng band(-1) and 30-1600 ng band(-1) ranges, respectively. Furthermore, the suggested HPTLC-UV methodology proved sensitive, accurate, precise, and robust for simultaneous detection of CZN and PCT. The assay of CZN in marketed capsules and tablets was found to be 99.01 +/- 1.53 and 100.87 +/- 1.61%, respectively, using the suggested HPTLC-UV method. The assay of PCT in commercial capsules and tablets was found to be 98.31 +/- 1.38 and 101.21 +/- 1.67%, respectively. The AGREE index for the greener HPTLC-UV technique was found to be 0.79, suggesting an excellent greenness profile for the proposed HPTLC-UV technique. These results and data suggested the suitability of the greener HPTLC-UV methodology for simultaneous estimation of CZN and PCT in commercial formulations.

Automated summary

This study developed a greener reverse-phase high-performance thin-layer chromatography with ultraviolet (HPTLC-UV) method for the simultaneous estimation of chlorzoxazone (CZN) and paracetamol (PCT) in commercial capsules and tablets. The proposed method showed excellent greenness profile and proved to be sensitive, accurate, precise, and robust for the simultaneous detection of CZN and PCT.