期刊
FOOD SCIENCE AND HUMAN WELLNESS
卷 11, 期 6, 页码 1515-1526出版社
KEAI PUBLISHING LTD
DOI: 10.1016/j.fshw.2022.06.009
关键词
Lymphocytes; Peptides; Lipopolysaccharide; Dipeptidyl peptidase IV (DPP-IV) inhibitory activity
The objective of this study was to isolate and identify intracellular bioactive peptides from mouse lymphocytes before and after lipopolysaccharide (LPS) stimulation. A total of 131 novel peptides were discovered, and their bioactivity and function were predicted. Two peptides with better inhibition activity were verified.
The objective of this study was to isolate and identify the intracellular bioactive peptides from mouse lymphocytes before and after lipopolysaccharide (LPS) stimulation, to explore novel peptides and to research the bioactive function. Mouse spleen lymphocytes were isolated and cultured with LPS stimulation (experimental group) or not (control group) to collect intracellular peptides. Totally 385 peptides were analyzed by nanoliter liquid phase-Q Exactive quadrupole ultra-high resolution orbitrap mass spectrometer (Nano LC-Q Exactive Plus) and identified by PEAKS X software. After compared with peptides reported, 131 novel peptides were discovered, which then were predicted bioactivity by Peptide Ranker and 6 peptides with high bioactivity were predicted function by BIOPEP-UMW database. Prediction data showed that they may have dipeptidyl peptidase IV (DPP-IV) inhibitory activity. Finally, two peptides showed better potent inhibition were verified with competitive and noncompetitive modes.(c) 2022 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据