期刊
LIFE-BASEL
卷 12, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/life12111749
关键词
gut microbiota; Micrococcus porci; new taxa; polyphasic analysis
资金
- Council of Agriculture, Taiwan, ROC
- COA [111AS-2.1.1-LI-L6]
- Ministry of Economic Affairs, Taiwan, ROC [111-EC-17-A-22-0525]
A new species of Micrococcus, named Micrococcus porci, was isolated from the fecal samples of a black pig. It exhibits spherical, non-motile, and non-spore-forming cells.
An aerobic bacterium, designated as strain KD337-16(T), was isolated from the fecal samples of a black pig. It exhibited spherical, non-motile and non-spore-forming, Gram-positive cells. KD337-16(T) was identified as a member of the genus Micrococcus through 16S rRNA gene sequencing, and its closest relatives were found to be Micrococcus endophyticus YIM 56238(T) (99.5% similarity), Micrococcus luteus NCTC 2665(T) (99.1%), Micrococcus yunnanensis YIM 65004(T) (99.1%), Micrococcus aloeverae AE-6(T) (99.1%), Micrococcus antarcticus T2(T) (98.9%), and Micrococcus flavus LW4(T) (98.7%). Phylogenomic trees were constructed, and strain KD337-16(T) was found to form its own cluster as an independent lineage of M. flavus LW4(T). Between KD337-16(T) and its close relatives, the average nucleotide identity, average amino acid identity, and digital DNA-DNA hybridization were below the respective species delineation thresholds at 82.1-86.6%, 78.1-86.1%, and 24.4-34.9%. The major cellular fatty acids and polar lipids were anteiso-C-15:0 and iso-C-15:0, and DPG and PG, respectively. The predominant menaquinone was MK-8(H-2). Taken together, the results indicate that strain KD337-16(T) is a novel species of the genus Micrococcus, for which the name Micrococcus porci sp. nov. is proposed. The type strain is KD337-16(T) (=BCRC 81318(T) = NBRC 115578(T)).
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