New Aspects of Bilayer Lipid Membranes for the Analysis of Ion Channel Functions

The bilayer lipid membrane (BLM) is the main structural component of cell membranes, in which various membrane proteins are embedded. Artificially formed BLMs have been used as a platform in studies of the functions of membrane proteins, including various ion channels. In this review, we summarize recent advances that have been made on artificial BLM systems for the analysis of ion channel functions. We focus on two BLM-based systems, cell-membrane mimicry and four-terminal BLM systems. As a cell-membrane-mimicking system, an efficient screening platform for the evaluation of drug side effects that act on a cell-free synthesized channel has been developed, and its prospects for use in personalized medicine will be discussed. In the four-terminal BLMs, we introduce lateral voltage to BLM systems as a novel input to regulate channel activities, in addition to the traditional transmembrane voltages. Such state-of-the-art technologies and new system setups are predicted to pave the way for a variety of applications, in both fundamental physiology and in drug discovery.

Automated summary

This review discusses the applications of artificial bilayer lipid membrane (BLM) systems in the analysis of ion channel functions. It focuses on two BLM-based systems, cell-membrane mimicry and four-terminal BLM systems. The cell-membrane mimicry system can be used for evaluating drug side effects and has potential in personalized medicine. The four-terminal BLM system introduces lateral voltage as a novel input to regulate channel activities.