Fouling Analysis in One-Stage Ultrafiltration of Precipitation-Treated Bacillus subtilis Fermentation Liquors for Biosurfactant Recovery

Primary recovery of surfactin from precipitation-pretreated fermentation broths of Bacillus subtilis ATCC 21332 culture by one-stage dead-end and cross-flow ultrafiltration (UF) was studied. Dead-end experiments were first performed to select suitable conditions, including the amount of added ethanol-a micelle-destabilizing solvent (0-70 vol%), type (polyethersulfone, polyacrylonitrile, poly(vinylidene fluoride)) and molecular-weight cut-off (MWCO, 30-100 kDa) of the membrane in the surfactin concentration range of 0.25-1.23 g/L. Then, the cross-flow UF experiments were conducted to check the recovery performance in the ranges of feed surfactin concentration of 1.13-2.67 g/L, flow velocity of 0.025-0.05 m/s, and transmembrane pressure of 40-100 kPa. The Hermia model was also used to clarify membrane fouling mechanisms. Finally, three cleaning agents and two in situ cleaning ways (flush and back-flush) were selected to regain the permeate flux. As for the primary recovery of surfactin from the permeate in cross-flow UF, a polyethersulfone membrane with 100-kDa MWCO was suggested, and the NaOH solution at pH 11 was used for membrane flushing.

Automated summary

This study investigated the primary recovery of surfactin from precipitation-pretreated fermentation broths using one-stage dead-end and cross-flow ultrafiltration. The results showed that the use of a polyethersulfone membrane and addition of ethanol were effective in recovering surfactin under suitable conditions. The Hermia model was used to understand membrane fouling mechanisms, and appropriate cleaning agents and methods were selected to restore membrane flux.