期刊
BIOSENSORS-BASEL
卷 12, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/bios12100791
关键词
2-arachidonoylglycerol; sandwich-type immunosensor; gold nanoparticles; biomedical electroanalysis; biomedicine; polymeric interface
资金
- Tabriz University of Medical Sciences
In this study, a new sandwich-type electrochemical immune device was used to detect 2-AG in human plasma. The use of poly-beta-cyclodextrin functionalized with AuNPs-DDT and toluidine blue improved the activity and stability of the immobilized 2-AG antibody. The developed immunosensor showed high sensitivity and accuracy, making it suitable for the diagnosis of 2-AG.
In this work, 2-AG was successfully detected in human plasma samples using a new sandwich-type electrochemical immune device based on poly-beta-cyclodextrin P(beta-CD) functionalized with AuNPs-DDT and toluidine blue. The P(beta-CD) ensured the bioactivity and stability of the immobilized 2-AG antibody by providing a broad surface for the efficient immobilization of the biotinylated antibody. To complete the top section of the immunosensor (reporter), an HRP-conjugated antibody of 2-AG (secondary antibody (Ab2)) was attached to the surface of a glassy carbon electrode (GCE) modified by P(beta-CD), as well as a primarily biotinylated antibody (Ab1). The biosensor fabrication process was monitored using field-emission scanning electron microscope (FE-SEM) and EDS methods. Using the differential pulse voltammetry technique, the immunosensor was utilized for detection of 2-AG in real samples. The suggested interface increased the surface area, which allowed for the immobilization of a large quantity of anti-2-AG antibody while also improving biocompatibility, stability, and electrical conductivity. Finally, the suggested immunosensor's limit of quantitation was determined to be 0.0078 ng/L, with a linear range of 0.0078 to 1.0 ng/L. The results showed that the suggested bioassay can be utilized for diagnosis of 2-AG in clinical samples as a unique and ultrasensitive electrochemical biodevice.
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