4.7 Article

A Molecular Lateral Flow Assay for SARS-CoV-2 Quantitative Detection

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BIOSENSORS-BASEL
卷 12, 期 11, 页码 -

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MDPI
DOI: 10.3390/bios12110926

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COVID-19; coronavirus; rapid test; gold nanoparticles; internal standard; strip

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A novel gold-nanoparticle-based nucleic acid lateral flow assay was developed for the rapid, visual, and quantitative detection of SARS-CoV-2. This method offers rapid, accurate, and cost-effective detection, making it a promising future diagnostic tool.
Since the onset of the SARS-CoV-2 pandemic, several COVID-19 detection methods, both commercially available and in the lab, have been developed using different biomolecules as analytes and different detection and sampling methods with high analytical performance. Developing novel COVID-19 detection assays is an exciting research field, as rapid accurate diagnosis is a valuable tool to control the current pandemic, and also because the acquired knowledge can be deployed for facing future infectious outbreaks. We here developed a novel gold-nanoparticle-based nucleic acid lateral flow assay for the rapid, visual, and quantitative detection of SARS-CoV-2. Our method was based on the use of a DNA internal standard (competitor) for quantification and involved RT-PCR, the hybridization of biotinylated PCR products to specific oligonucleotide probes, and detection with a dual lateral flow assay using gold nanoparticles conjugated to an anti-biotin antibody as reporters. The developed test allowed for rapid detection by the naked eye and the simultaneous quantification of SARS-CoV-2 in nasopharyngeal swabs with high specificity, detectability, and repeatability. This novel molecular strip test for COVID-19 detection represents a simple, cost-effective, and accurate rapid test that is very promising to be used as a future diagnostic tool.

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