4.7 Article

A Luciferase Mutant with Improved Brightness and Stability for Whole-Cell Bioluminescent Biosensors and In Vitro Biosensing

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Review Chemistry, Analytical

Portable light detectors for bioluminescence biosensing applications: A comprehensive review from the analytical chemist's perspective

Maria Maddalena Calabretta et al.

Summary: Bioluminescence is extensively used in various bioanalytical applications, and the advancement of portable light detectors has made it possible to integrate bioluminescence detection into miniaturized devices. Different photon detectors, such as PMTs, CCDs, CMOS, SPADs, SiPMs, and smartphone-integrated CMOS, have their pros and cons, and the selection of the most suitable detector should take into account factors such as instrumental noise, power supply, imaging capability, and ease of integration.

ANALYTICA CHIMICA ACTA (2022)

Article Biology

Firefly genomes illuminate the evolution of beetle bioluminescent systems

Yuichi Oba et al.

Summary: Fireflies, well-known bioluminescent organisms, have been extensively studied for their reaction mechanism and ecological functions. The genome assemblies of six species of bioluminescent beetles have recently been published, focusing on the evolution of novelties, luciferase, luciferin biosynthesis, and defensive chemicals. Candidate genes for critical roles in beetle bioluminescence have been identified, but functional analyses are ongoing. The establishment of a long-term mass-rearing system and strain will be crucial for post-genome research on bioluminescent beetles. Applying contemporary chromosome-scale genome assembly techniques to luminous beetles can help resolve outstanding evolutionary questions.

CURRENT OPINION IN INSECT SCIENCE (2022)

Article Chemistry, Analytical

Bioluminescence Sensing in 3D Spherical Microtissues for Multiple Bioactivity Analysis of Environmental Samples

Maria Maddalena Calabretta et al.

Summary: The development of predictive in vitro sensing tools for rapid information on bioactivities is crucial for monitoring environmental toxicants and understanding their mechanisms of action. We have developed a simple bioluminescent multiplexed assay that can measure four bioactivities and successfully applied it to the analysis of river water samples, showing potential applicability for environmental analyses.

SENSORS (2022)

Article Biochemistry & Molecular Biology

UCSF ChimeraX: Structure visualization for researchers, educators, and developers

Eric F. Pettersen et al.

Summary: UCSF ChimeraX is a powerful visualization program with enhanced performance and graphics, offering new tools and analysis features, support for various areas like virtual reality, and improved ease of use, along with an app store for researchers to contribute new tools.

PROTEIN SCIENCE (2021)

Article Biophysics

Orthogonal paper biosensor for mercury(II) combining bioluminescence and colorimetric smartphone detection

Antonia Lopreside et al.

Summary: This low-cost biosensor integrates three different biorecognition principles for orthogonal detection of mercury(II) on a three-leaf paper, providing a stable disposable device that can easily be inserted into a smartphone for mercury detection in water samples, with a detection limit reaching ppb levels.

BIOSENSORS & BIOELECTRONICS (2021)

Article Engineering, Environmental

Improved Aliivibrio fischeri based-toxicity assay: Graphene-oxide as a sensitivity booster with a mobile-phone application

Jose Francisco Bergua et al.

Summary: This study developed a mobile-phone-based sensing platform for water toxicity assessment in just 5 minutes using two common pesticides as model analytes. New methodologies were established to enhance the bioluminescent signal of A. fischeri, and the biocompatibility of graphene oxide to the bacterium was investigated to improve the sensitivity of toxicity assays and bacterial growth.

JOURNAL OF HAZARDOUS MATERIALS (2021)

Article Chemistry, Analytical

A Genetically Encoded Bioluminescence Intracellular Nanosensor for Androgen Receptor Activation Monitoring in 3D Cell Models

Maria Maddalena Calabretta et al.

Summary: This study introduces an intracellular nanosensor for screening compounds with androgenic activity based on split NanoLuc reporter. The use of 3D spheroids of HEK-293 cells was confirmed to be suitable for upgrading the 2D cell-based assay.

SENSORS (2021)

Article Chemistry, Analytical

Ultrasensitive On-Field Luminescence Detection Using a Low-Cost Silicon Photomultiplier Device

Maria Maddalena Calabretta et al.

Summary: The study developed a portable device for detecting biochemiluminescence using SiPM technology, showing superior detection performance compared to traditional benchtop devices and smartphone sensors. Comparison of two model systems demonstrated the device's high sensitivity to low light intensities.

ANALYTICAL CHEMISTRY (2021)

Review Chemistry, Multidisciplinary

Applications of bioluminescence in biotechnology and beyond

Aisha J. Syed et al.

Summary: Bioluminescence is a natural phenomenon where living creatures produce light through a reaction between luciferin and luciferase. Various luciferin-luciferase pairs have different applications, such as gene assays and drug discovery. In recent years, bioluminescence has found increasing applications in biomedicine, including photodynamic therapy and neuron control.

CHEMICAL SOCIETY REVIEWS (2021)

Article Biophysics

Nano-lantern on paper for smartphone-based ATP detection

Maria Maddalena Calabretta et al.

BIOSENSORS & BIOELECTRONICS (2020)

Article Multidisciplinary Sciences

A new brilliantly blue-emitting luciferin-luciferase system from Orfelia fultoni and Keroplatinae (Diptera)

Vadim R. Viviani et al.

SCIENTIFIC REPORTS (2020)

Article Biochemical Research Methods

Pret-a-porter nanoYESα and nanoYESβ bioluminescent cell biosensors for ultrarapid and sensitive screening of endocrine-disrupting chemicals

Antonia Lopreside et al.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2019)

Review Biotechnology & Applied Microbiology

NanoBRET: The Bright Future of Proximity-Based Assays

Natasha C. Dale et al.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2019)

Article Chemistry, Multidisciplinary

Firefly Luciferase Mutant with Enhanced Activity and Thermostability

Tania Pozzo et al.

ACS OMEGA (2018)

Article Biotechnology & Applied Microbiology

Surface charge modification increases firefly luciferase rigidity without alteration in bioluminescence spectra

Mojtaba Mortazavi et al.

ENZYME AND MICROBIAL TECHNOLOGY (2017)

Article Biochemistry & Molecular Biology

Bioluminescence Imaging of Spheroids for High-throughput Longitudinal Studies on 3D Cell Culture Models

Luca Cevenini et al.

PHOTOCHEMISTRY AND PHOTOBIOLOGY (2017)

Article Biochemical Research Methods

Red-emitting chimeric firefly luciferase for in vivo imaging in low ATP cellular environments

Bruce R. Branchini et al.

ANALYTICAL BIOCHEMISTRY (2017)

Article Multidisciplinary Sciences

Near-infrared bioluminescent proteins for two-color multimodal imaging

Konstantin A. Rumyantsev et al.

SCIENTIFIC REPORTS (2016)

Article Chemistry, Multidisciplinary

Experimental Support for a Single Electron-Transfer Oxidation Mechanism in Firefly Bioluminescence

Bruce R. Branchini et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2015)

Review Cell Biology

3D Cell Culture Systems: Advantages and Applications

Maddaly Ravi et al.

JOURNAL OF CELLULAR PHYSIOLOGY (2015)

Article Biochemical Research Methods

Analytical strategies for improving the robustness and reproducibility of bioluminescent microbial bioreporters

Aldo Roda et al.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2011)

Article Biochemistry & Molecular Biology

Design of thermostable luciferases through arginine saturation in solvent-exposed loops

Mojtaba Mortazavi et al.

PROTEIN ENGINEERING DESIGN & SELECTION (2011)

Article Biochemical Research Methods

Red-emitting luciferases for bioluminescence reporter and imaging applications

Bruce R. Branchini et al.

ANALYTICAL BIOCHEMISTRY (2010)

Article Biochemistry & Molecular Biology

The effective role of positive charge saturation in bioluminescence color and thermostability of firefly luciferase

Bagher Said Alipour et al.

PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES (2009)

Article Biochemical Research Methods

Thermostable red and green light-producing firefly luciferase mutants for bioluminescent reporter applications

Bruce R. Branchini et al.

ANALYTICAL BIOCHEMISTRY (2007)

Article Biochemistry & Molecular Biology

Mutagenesis of solvent-exposed amino acids in Photinus pyralis luciferase improves thermostability and pH-tolerance

G. H. Erica Law et al.

BIOCHEMICAL JOURNAL (2006)

Article Biochemical Research Methods

Thermostability of Firefly Luciferases Affects Efficiency of Detection by In Vivo Bioluminescence

Brenda Baggett et al.

MOLECULAR IMAGING (2004)

Review Biochemistry & Molecular Biology

The origin, diversity, and structure function relationships of insect luciferases

VR Viviani

CELLULAR AND MOLECULAR LIFE SCIENCES (2002)