期刊
JOURNAL OF MICROENCAPSULATION
卷 33, 期 6, 页码 569-574出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/02652048.2016.1228703
关键词
Bile-acid based microcapsules; beta-cells; biomaterials; inflammation; insulin
资金
- Australian Postgraduate Award
- Curtin Research Scholarship
Objective: In a recent study, we developed a new microencapsulating method for beta-cell microencapsulation, but cell viability declined rapidly, post microencapsulation, due to potential polymer-polyelectrolyte chelation and non-porous microcapsules' membranes resulting in cell apoptosis. Thus, this study tested the effects of incorporating cationic polyamine at 1% w/v, on microcapsule strength and cell viability, in the absence or presence of an anionic tertiary bile acid (ATBA) with potential cell-protective effects.Methods: 1% w/v polyamine was used without or with ATBA, to form beta-cell microcapsules and physical and biological analyses was carried out 50h post microencapsulation.Results: Microcapsules containing 1% w/v polyamine showed weak physical properties and low cell viability and ATBA incorporation resulted in >30% reduction in cell viability and increased levels of pro-inflammatory cytokines.Conclusion: Neither 1% w/v polyamine nor the presence of ATBA resulted in optimised cell viability, but rather reduced cell viability, enhanced inflammation and lowered insulin secretion.
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