期刊
FRONTIERS IN NUTRITION
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fnut.2022.1001334
关键词
blackened jujube; polysaccharides; structural features; antitumor activity; mechanism
资金
- Central Guidance on Local Science and Technology Development Program [YDZX2021071]
- Shandong Province Key Research and Development Program (Rural revitalization of science and technology innovation boosting action) [2021TZXD011]
- Dezhou Health Food Industry Innovation and Entrepreneurship Community
- Shandong Land and Rural Revitalization Group Co., Ltd.
This study extracted a novel acidic polysaccharide BJP-2 from blackened jujube and analyzed its chemical characteristics. The results showed that BJP-2 could inhibit cancer cell proliferation by inducing apoptosis and block cancer cell invasion and metastasis. Further protein analysis revealed that BJP-2 exhibited anti-tumor activity through the mitochondria-dependent pathway.
Liver cancer is one of the most common cancers, with increasing trends in incidence and mortality. A novel acidic polysaccharide (BJP-2) obtained from blackened jujube was extracted by hot water followed by chromatographic purification employing DEAE-cellulose 52 and Sephadex G-100 column. And then BJP-2 was identified by SEC-MALLS-RI, GC-MS, methylation and NMR for the following characteristics: molecular weight of 6.42 x 10(4) Da, monosaccharide composition of glucuronic acid (GalA), arabinose (Ara), galactose (Gal), rhamnose (Rha), xylose (Xyl), glucuronic acid (GlcA), glucose (Glc), fucose (Fuc) and mannose (Man) with the percentage of 39.78, 31.93, 16.86, 6.43, 1.86, 1.28, 1.02, 0.61, and 0.23%, as well as the main chain of -> 5)-alpha-L-Araf (1 -> 4)-beta-D-Gal(1 -> , T-alpha-L-Araf (1 -> 4)-beta-D-Gal(1 -> , and -> 4)-alpha-L-6MeGalAp(1 -> . The effect of BJP-2 on the apoptosis of HepG2 cells and its anti-tumor mechanism were further explored. The analysis by MTT and flow cytometry showed that BJP-2 suppressed cell proliferation by inducing apoptosis in a concentration-dependent manner. Cell scratching and Transwell revealed that BJP-2 was able to block the invasion and metastasis of tumor cells. Western blot results demonstrated that BJP-2 exhibited antitumor activity through a mitochondria-dependent pathway, as evidenced by overexpression of Bax, Cleaved Caspase-3/Caspase-3 and Cleaved Caspase-9/Caspase-9 and downregulation of Bcl-2. Therefore, BJP-2 has broad research prospects as a tumor preventive or therapeutic agent.
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