期刊
KIDNEY INTERNATIONAL REPORTS
卷 8, 期 2, 页码 317-329出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.ekir.2022.10.024
关键词
ACTN4; Drosophila; FSGS; nephrocyte; nephrotic syndrome; podocyte
This study reports a case of a 4-year-old boy with proteinuria and biopsy-proven focal segmental glomerulosclerosis (FSGS). Molecular genetic testing identified a novel mutation in alpha-actinin-4 that is associated with the disease. The study showed that the mutation led to decreased stability of alpha-actinin-4, protein mislocalization, and cytoskeleton rearrangements. Using a Drosophila model, the researchers demonstrated the pathogenicity of the mutation.
Introduction: Genetic disorders are among the most prevalent causes leading to progressive glomerular disease and, ultimately, end-stage renal disease (ESRD) in children and adolescents. Identification of underlying genetic causes is indispensable for targeted treatment strategies and counseling of affected patients and their families. Methods: Here, we report on a boy who presented at 4 years of age with proteinuria and biopsy-proven focal segmental glomerulosclerosis (FSGS) that was temporarily responsive to treatment with ciclo-sporin A. Molecular genetic testing identified a novel mutation in alpha-actinin-4 (p.M240T). We describe a feasible and efficient experimental approach to test its pathogenicity by combining in silico, in vitro, and in vivo analyses. Results: The de novo p.M240T mutation led to decreased alpha-actinin-4 stability as well as protein mislocalization and actin cytoskeleton rearrangements. Transgenic expression of wild-type human alpha-actinin-4 in Drosophila melanogaster nephrocytes was able to ameliorate phenotypes associated with the knockdown of endogenous actinin. In contrast, p.M240T, as well as other established disease variants p.W59R and p.K255E, failed to rescue these phenotypes, underlining the pathogenicity of the novel alpha-actinin-4 variant. Conclusion: Our data highlight that the newly identified alpha-actinin-4 mutation indeed encodes for a disease-causing variant of the protein and promote the Drosophila model as a simple and convenient tool to study monogenic kidney disease in vivo.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据