期刊
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
卷 49, 期 4, 页码 494-501出版社
ELSEVIER TAIWAN
DOI: 10.1016/j.jmii.2014.08.023
关键词
beta-lactamase-producing Klebsiella pneumonia; Klebsiella pneumoniae; regulator of mucoid phenotype A
资金
- National Health Research Institutes
- Medical Foundation in Memory of Dr. Deh-Lin Cheng
- Tri-Service General Hospital, Taipei, Taiwan [TSGH-C102-123, TSGH-C102-124, DOD103-M078, DOD103-M079, DOD103-M080]
- National Science Council, Taipei, Taiwan [NSC101-2314-B-016-022-MY3]
Background: To determine whether the presence of a capsule regulator gene [i.e., regulator of mucoid phenotype A (rmpA) gene] contributes to virulence on extended-spectrum beta-lactamase-producing Klebsiella pneumoniae (ESBL-KP) with serotype non-K1/K2 strains. Methods: Twenty-eight ESBL-KP and non-ESBL-KP isolates were collected from the Tri-Service General Hospital (Taipei, Taiwan). The impact of the virulent rmpA gene in different capsular polysaccharide serotypes on ESBL-KP and non-ESBL-KP isolates was studied by a neutrophil phagocytosis reaction, a serum bactericidal assay, and an animal survival model. Results: Resistance to broad spectrum antibiotics was more prevalent in ESBL-KP strains than in non-ESBL-KP strains (p < 0.01). The ESBL-KP strains had different molecular patterns from non-ESBL-KP strains, based on pulsed-field gel electrophoresis. The frequency of serumresistant isolates was the highest among ESBL-KP strains with rmpA (i.e., rmpA(+)) [71.4% (5/7)] than among of non-ESBL-KP rmpA(+) strains [42.8% (6/14)], ESBL-KP strains without rmpA (rmpA(-)) [33.3% (7/21)], and non-ESBL-KP rmpA(-) strains [14.2% (2/14)]. The most significant increase in neutrophil resistance occurred in the ESBL-KP rmpA(+) strains in comparison to the non-ESBL-KP rmpA(+), ESBL-KP rmpA(+), and non-ESBL-KP rmpA(-) strains (p < 0.01). The results of the animal survival model were compatible with the neutrophil phagocytosis reaction and serum bactericidal assay. Conclusion: We conclude that the pathogenic potential is greater in rmpA(+) ESBL-KP strains than in rmpA(-) ESBL-KP and non-ESBL-KP strains. Copyright (C) 2014, Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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